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Image Search Results
Journal: CNS Neuroscience & Therapeutics
Article Title: Asparagine Endopeptidase Inhibition Attenuates Tissue Plasminogen Activator‐Induced Brain Hemorrhagic Transformation After Ischemic Stroke
doi: 10.1111/cns.70345
Figure Lengend Snippet: AEP KO ameliorates tPA‐associated hemorrhagic transformation in the stroke mouse model. (A, B) Representative images of hematoma in brain slices and quantification of hemoglobin levels. Data are presented as mean ± SEM and statistical analyses are performed using Welch test followed by Dunnett T3 multiple comparisons test were applied since the P value of Levene test < 0.05. (C) Recorded brain water content to assess brain edema at 24 h after delayed tPA administration. Data are presented as mean ± SEM and statistical analysis is performed using one‐way ANOVA test followed by Tukey's multiple comparisons test. (D) ELISA for tPA activity detection. Data are presented as mean ± SEM and statistical analyses are performed using Welch test followed by Dunnett T3 multiple comparisons test were applied since the P value of Levene test < 0.05. (E) Representative images of HE staining in the cortical hematoma area (scale bar = 50 μm). (A), (C) n = 6, (D) n = 4 per group. Normality and variance are assessed via Shapiro‐Wilk test and Levene's test, respectively. * P < 0.05, ** P < 0.01.
Article Snippet: A
Techniques: Transformation Assay, Enzyme-linked Immunosorbent Assay, Activity Assay, Staining
Journal: CNS Neuroscience & Therapeutics
Article Title: Asparagine Endopeptidase Inhibition Attenuates Tissue Plasminogen Activator‐Induced Brain Hemorrhagic Transformation After Ischemic Stroke
doi: 10.1111/cns.70345
Figure Lengend Snippet: The 7,8‐DHF prodrug R13 confers protection in mice against neurological dysfunction, hemorrhagic transformation, and BBB disruption after cerebral ischemia and tPA treatment. Mice were given R13 (dissolved in 5% DMSO/0.5% methylcellulose) at a dose of 21.8 mg/kg/day, 7 days per week, for 2 weeks by gavage, followed by MCAO/R. Surgery and delayed tPA treatment. (A, B) Representative images of TTC staining and the corresponding proportion of the infarct area. (C, D) Representative images of hematoma in brain slices and quantification of hemoglobin levels. (E, F) Representative images and leakage quantification of Evans Blue staining. (G, H) Longa Scores and Corner Turn tests used for sensorimotor function examination. (I) ELISA for tPA activity detection. (A), (C), (F), (I) n = 4, (G, H) n = 20 per group. Data are presented as mean ± SEM, and statistical analyses are performed using one‐way ANOVA test followed by Tukey's multiple comparisons test when P value of Levene test > 0.05 or Welch test followed by Dunnett T3 multiple comparisons test when the P value of Levene test < 0.05. Normality and variance are assessed via Shapiro‐Wilk test and Levene's test, respectively. * P < 0.05, ** P < 0.01.
Article Snippet: A
Techniques: Transformation Assay, Disruption, Staining, Enzyme-linked Immunosorbent Assay, Activity Assay